APOE antibody (Isoform E3) (Biotin)

Catalog No. ABIN181665

Product Details anti-APOE Antibody (Biotin)

Target: APOE (Apolipoprotein E (APOE))

Binding Specificity: Isoform E3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOE antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot.

Purification: Affinity Chromatography.

Immunogen: E.Coli derived recombinant Human ApoE3.

Application Details

Application Notes: Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 μL/well antibody solution)a concentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated antibody, inconjunction with Affinity purified anti-Human ApoE3 (ABIN181667 or ABIN181668) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanApoE3. Western blot: To detect Human ApoE3 this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: Apolipoprotein E (Apo E) (APOE Products)

Synonyms: ad2 antibody, apoprotein antibody, im:7036787 antibody, wu:fb69a05 antibody, zgc:110064 antibody, apoe antibody, AI255918 antibody, AD2 antibody, LDLCQ5 antibody, LPG antibody, APOEA antibody, Apo-E antibody, apolipoprotein E antibody, apolipoprotein Ea antibody, apoe antibody, apoea antibody, Apoe antibody, APOE antibody

Background: Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues.Synonyms: Apo-E, ApoE

Gene ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism