AKT1 antibody (AA 5-30)

Catalog No. ABIN1821257

The Mouse Monoclonal anti-AKT1 antibody is suitable to detect AKT1 in samples from Human. It has been validated for WB and ICC.

Quick Overview for AKT1 antibody (AA 5-30) (ABIN1821257)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: 1D4B

Product Details anti-AKT1 Antibody

Binding Specificity: AA 5-30

Specificity: Human Akt1. Does not react with 293-EBNA human EBV-expressing embryonic kidney cell line transfectant expressing wild type AKT1.

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to aa5-30 from human Akt1 with Glu17Lys mutation.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ICC (8 - 25 μg/mL), WB (0.5 μg/mL)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS to 0.5 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, trehalose

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1 / AKT

Background: Name/Gene ID: AKT1
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA

Gene ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect