MAPKAP Kinase 2 antibody (AA 44-55)
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- Target See all MAPKAP Kinase 2 (MAPKAPK2) Antibodies
- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
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Binding Specificity
- AA 44-55
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Reactivity
- Human, Mouse, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This MAPKAP Kinase 2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-PQFHVKSGLQIK from an internal region of human MAPKAPK2 / MK2 (NP_004750.1, NP_116584.2). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey (100%), Galago, Mouse, Rat, Panda, Rabbit, Pig, Guinea pig (92%), Bovine, Opossum (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MAPKAPK2 Primary Antibody
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- Application Notes
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Approved: Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 μg/mL. Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 μg EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
- Alternative Name
- MAPKAPK2 / MK2 (MAPKAPK2 Products)
- Synonyms
- mapkapk2 antibody, wu:fb30e10 antibody, wu:fc56c10 antibody, wu:fi03a01 antibody, MGC78852 antibody, MAPKAPK2 antibody, AA960234 antibody, MAPKAP-K2 antibody, MK-2 antibody, MK2 antibody, Rps6kc1 antibody, mitogen-activated protein kinase-activated protein kinase 2a antibody, mitogen-activated protein kinase-activated protein kinase 2 L homeolog antibody, mitogen-activated protein kinase-activated protein kinase 2 antibody, MAP kinase-activated protein kinase 2 antibody, mapkapk2a antibody, mapkapk2.L antibody, MAPKAPK2 antibody, Mapkapk2 antibody
- Background
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Name/Gene ID: MAPKAPK2
Subfamily: MAPKAPK
Family: Protein Kinase
Synonyms: MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2 - Gene ID
- 9261
- NCBI Accession
- NP_004750, NP_116584
- UniProt
- P49137
- Pathways
- MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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