MAPKAP Kinase 2 antibody (AA 44-55)

Catalog No. ABIN1833999

Product Details anti-MAPKAP Kinase 2 Antibody

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Binding Specificity: AA 44-55

Reactivity: Human, Mouse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPKAP Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PQFHVKSGLQIK from an internal region of human MAPKAPK2 / MK2 (NP_004750.1, NP_116584.2). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey (100%), Galago, Mouse, Rat, Panda, Rabbit, Pig, Guinea pig (92%), Bovine, Opossum (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 μg/mL. Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 μg EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Alternative Name: MAPKAPK2 / MK2 (MAPKAPK2 Products)

Synonyms: mapkapk2 antibody, wu:fb30e10 antibody, wu:fc56c10 antibody, wu:fi03a01 antibody, MGC78852 antibody, MAPKAPK2 antibody, AA960234 antibody, MAPKAP-K2 antibody, MK-2 antibody, MK2 antibody, Rps6kc1 antibody, mitogen-activated protein kinase-activated protein kinase 2a antibody, mitogen-activated protein kinase-activated protein kinase 2 L homeolog antibody, mitogen-activated protein kinase-activated protein kinase 2 antibody, MAP kinase-activated protein kinase 2 antibody, mapkapk2a antibody, mapkapk2.L antibody, MAPKAPK2 antibody, Mapkapk2 antibody

Background: Name/Gene ID: MAPKAPK2
Subfamily: MAPKAPK
Family: Protein Kinase

Synonyms: MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2

Gene ID: 9261

NCBI Accession: NP_004750, NP_116584

UniProt: P49137

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades