LIME antibody (AA 281-296)

Catalog No. ABIN1839558

Product Details anti-LIME Antibody

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Binding Specificity: AA 281-296

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIME antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Purification: Purified

Immunogen: COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.

Type of Immunogen: Hapten

Clone: LIME-10

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: LIME1 / LIME (LIME1 Products)

Synonyms: LIME1 antibody, DKFZp459A158 antibody, LIME antibody, LP8067 antibody, dJ583P15.4 antibody, 2810038K19Rik antibody, RGD1562795 antibody, Lck interacting transmembrane adaptor 1 antibody, LIME1 antibody, Lime1 antibody

Background: Name/Gene ID: LIME1

Synonyms: LIME1, DJ583P15.4, Lck-interacting molecule, LIME, RP4-583P15.5, LP8067

Gene ID: 54923

UniProt: Q9H400