Rabbit Anti-NF-kappaB p100 Polyclonal Antibody detects endogenous levels of total NF-kappaB p100 protein.
Characteristics
Molecular Weight: Predicted Band Size 120 kDa, Observed Band Size 120 kDa
Purification
Rabbit Anti-NF-kappaB p100 (Ab-870) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Immunogen
Synthesized non-phosphopeptide derived from human NF-kappaB p100 around pSer870 (Y-G-SP-Q-S)
NFKB2
Reactivity: Human, Mouse
ELISA, IHC (p), ICC, FACS, IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500-1:1,000, IHC: 1:50-1:100
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store at -20 °C for 1 year.
Chang, Al-Alwan, Risse, Halayko, Martin, Baglole, Eidelman, Hamid: "Th17-associated cytokines promote human airway smooth muscle cell proliferation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 12, pp. 5152-60, (2012) (PubMed).
Target
NFKB2
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 2 (NFKB2))