NF-kB p65 antibody (pSer529)
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- Target See all NF-kB p65 (NFkBP65) Antibodies
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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Binding Specificity
- pSer529
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NF-kB p65 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- Detects endogenous levels of NF-kappa,B p65 protein only when phosphorylated at serine 529.
- Characteristics
- Molecular Weight: Predicted Band Size 65 kDa, Observed Band Size 65 kDa
- Purification
- The antibody is affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Synthesized phosphopeptide derived from human NF-kappa,B p65 around pSer529 (L-L-SP-G-D)
- Isotype
- IgG
- Top Product
- Discover our top product NFkBP65 Primary Antibody
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- Application Notes
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Western blot: 1:500-1:1,000,
IHC: 1:50-1:100,
IF: 1:100-1:200 - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing. - Storage
- -20 °C
- Storage Comment
- Store the antibody at -20 °C. It will remain stable for one year.
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- Target
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- Alternative Name
- NF-KappaB p65 (NFkBP65 Products)
- Synonyms
- C-Rel antibody, NFKB3 antibody, p65 antibody, NFkB antibody, c-rel antibody, zgc:100833 antibody, Xrel2 antibody, Xrel3 antibody, rel antibody, rel-A antibody, rel2 antibody, rel3 antibody, v-rel antibody, xrel antibody, REL proto-oncogene, NF-kB subunit antibody, RELA proto-oncogene, NF-kB subunit antibody, v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, v-rel avian reticuloendotheliosis viral oncogene homolog antibody, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antibody, REL antibody, RELA antibody, Rela antibody, Rel antibody, rel antibody, rel.L antibody
- Pathways
- NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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