Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
Rabbit Anti-AMPKalpha,1 (Phospho-Ser487) Polyclonal Antibody detects endogenous levels of AMPKalpha,1 only when phosphorylated at serine 487.
Characteristics
Molecular Weight: Predicted Band Size 63 kDa, Observed Band Size 63 kDa
Purification
Rabbit Anti-AMPKalpha,1 (Phospho-Ser487) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Synthesized phosphopeptide derived from human AMPKalpha,1 around pSer487 (S-G-SP-V-S)
Western blot: 1:500-1:1,000, IHC: 1:50-1:100, IF: 1:100-1:200
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Avoid repeated freezing and thawing.
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.