HSF1 antibody (pSer307)

Catalog No. ABIN1842530

Product Details anti-HSF1 Antibody

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Binding Specificity: pSer307

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Rabbit Anti-HSF1 (Phospho-Ser307) antibody detects endogenous levels of HSF1 only when phosphorylated at serine 307.

Characteristics: Molecular Weight: Predicted Band Size 82 kDa, Observed Band Size 82 kDa

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human HSF1 around pSer307 (P-Q-SP-P-R).

Isotype: IgG

Application Details

Application Notes: Western blot: 1:500-1:1,000,
IF: 1:100-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.,
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The product remains stable for up to one year if stored at -20 °C.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1 (HSF1 Products)

Synonyms: hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody