FOXO3 antibody (C-Term)

Catalog No. ABIN185248

This Goat Polyclonal antibody specifically detects FOXO3 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for FOXO3 antibody (C-Term) (ABIN185248)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-FOXO3 Antibody

Binding Specificity: C-Term

Purpose: FOXO3A / FOXO3 / FKHRL1

Sequence: GAKQASSQSW VPG

Specificity: Both variants represent identical protein (NP_001446.1 and NP_963853.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GAKQASSQSWVPG, from the C Terminus of the protein sequence according to NP_001446.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70 kDa band observed in Human Heart lysates (calculated MW of 71.3 kDa according to NP_001446.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3

Background: FOXO3, FOXO3A, FKHRL1, AF6q21, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, forkhead box O3A, forkhead (Drosophila) homolog, FOXO2, forkhead homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 (rhabdomyosarcoma)

Gene ID: 2309, 56484

NCBI Accession: NP_001446

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis