IFNB1 antibody (AA 22-186) (Biotin)

Catalog No. ABIN1859274

This anti-IFNB1 antibody is a Rabbit Polyclonal antibody detecting IFNB1 in WB, IHC and ICC. Suitable for Pig.

Quick Overview for IFNB1 antibody (AA 22-186) (Biotin) (ABIN1859274)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-IFNB1 Antibody (Biotin)

Binding Specificity: AA 22-186

Purpose: Biotin-Linked Polyclonal Antibody to Interferon Beta (IFNb)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -MSYDVLRYQ QRSSNLACQK LLGQLPGTPQ YCLEDRMNFE VPEEIMQPPQ FQKEDAVLII HEMLQQIFGI LRRNFSSTGW NETVIKTILV ELDGQMDDLE TILEEIMEEE NFPRGDMTIL HLKKYYLSIL QYLKSKEYRS CAWTVVQVEI LRNFSFLNRL TDYLRN

Specificity: The antibody is a rabbit polyclonal antibody raised against IFNb. It has been selected for its ability to recognize IFNb in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant IFNb expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against IFNb conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IFNB1

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Alternative Name: Interferon Beta

Background: IFNB1, IFN-B, IFB, IFF, IFNB, Interferon Beta 1 Fibroblast

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome