TGFA antibody (AA 24-98)
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- Target See all TGFA Antibodies
- TGFA (Transforming Growth Factor, alpha (TGFA))
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Binding Specificity
- AA 24-98
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Reactivity
- Rabbit
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Host
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Guinea Pig
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Clonality
- Polyclonal
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Conjugate
- This TGFA antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Sequence
- MGHHHHHHSG SEF- ENSTSPL SDPPVAAAVV SHFNDCPDSH TQFCFHGTCR FLVQEDKPAC VCHSGYVGAR CEHADLLAVV AASQKKQA
- Specificity
- It has been selected for its ability to recognize TGFa in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant TGFa expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against TGFa. - Isotype
- IgG
- Top Product
- Discover our top product TGFA Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant TGFa (Glu24~Ala98) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- TGFA (Transforming Growth Factor, alpha (TGFA))
- Alternative Name
- Transforming Growth Factor Alpha (TGFA Products)
- Synonyms
- TFGA antibody, LOC100009482 antibody, TGF alpha antibody, TGFA antibody, wa-1 antibody, wa1 antibody, RATTGFAA antibody, TGFAA antibody, NV15606 antibody, si:ch211-10e8.7 antibody, transforming growth factor alpha antibody, transforming growth factor alpha L homeolog antibody, transforming growth factor-alpha antibody, transforming growth factor, alpha antibody, TGFA antibody, TGF-A antibody, Tgfa antibody, tgfa.L antibody, LOC100121300 antibody, LOC100009150 antibody, tgfa antibody
- Pathways
- NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway
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