LTA antibody (AA 49-194)
-
- Target See all LTA Antibodies
- LTA (Lymphotoxin-alpha (LTA))
-
Binding Specificity
- AA 49-194
-
Reactivity
- Rabbit
-
Host
-
Guinea Pig
-
Clonality
- Polyclonal
-
Conjugate
- This LTA antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Sequence
- MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GH STLKPAAHLV GDPSAQDSLR WRANTDRAFL RHGFSLSNNS LLVPSSGLYF VYSQVVFSGE GCSPKAVPTP LYLAHEVQLF SSQYSFHVPL LSAQKSVCPG PQGPWVRSVY QGAVFLLTQG DQLSTHTDGI AHLLLSPSSV FFGA
- Specificity
- It has been selected for its ability to recognize TNFb in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
- TNFb (AA 49-194)
- Isotype
- IgG
- Top Product
- Discover our top product LTA Primary Antibody
-
-
- Application Notes
-
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
-
Content: The quality control contains recombinant TNFb (Gly49~Ala194) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
-
- Target
- LTA (Lymphotoxin-alpha (LTA))
- Alternative Name
- Tumor Necrosis Factor Beta (TNFb) (LTA Products)
- Synonyms
- LT antibody, TNFB antibody, TNFSF1 antibody, LT-[a] antibody, LT-alpha antibody, LT[a] antibody, LTalpha antibody, Ltx antibody, TNF-beta antibody, Tnfb antibody, Tnfsf1b antibody, hlb382 antibody, lymphotoxin alpha antibody, lymphotoxin A antibody, LTA antibody, Lta antibody
- Target Type
- Chemical
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
-