ATP5J antibody (AA 1-108)

Catalog No. ABIN1867191

Product Details anti-ATP5J Antibody

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Binding Specificity: AA 1-108

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5J antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Coupling Factor 6 (CF6)

Specificity: The antibody is a rabbit polyclonal antibody raised against CF6. It has been selected for its ability to recognize CF6 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Coupling Factor 6 (CF6) corresdonding to Met1~Ala108 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Alternative Name: Coupling Factor 6 (ATP5J Products)

Synonyms: zgc:77541 antibody, wu:fa66e04 antibody, ATP5 antibody, ATP5A antibody, ATPM antibody, CF6 antibody, F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 L homeolog antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F antibody, atp5j antibody, ATP5J antibody, atp5j.L antibody, Atp5j antibody

Background: ATP5J, ATP5A, ATP5, ATPM, ATP Synthase,H+ Transporting,Mitochondrial Fo Complex,Subunit F6, ATP synthase-coupling factor 6, mitochondrial

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process