NFIB antibody (AA 1-233)
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- Target See all NFIB Antibodies
- NFIB (Nuclear Factor I/B (NFIB))
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Binding Specificity
- AA 1-233
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NFIB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Nuclear Factor I/B (NFIB)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against NFIB. It has been selected for its ability to recognize NFIB in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Nuclear Factor I/B (NFIB) corresdonding to Met1~Gln233 with N-terminal His and GST Tag
- Isotype
- IgG
- Top Product
- Discover our top product NFIB Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:250-1000 Immunohistochemistry: 5-20 μg/mL
1:25-100 Immunocytochemistry: 5-20 μg/mL
1:25-100 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- NFIB (Nuclear Factor I/B (NFIB))
- Alternative Name
- Nuclear Factor I/B (NFIB Products)
- Synonyms
- NFIB antibody, nf1-b1 antibody, CTF antibody, HMGIC/NFIB antibody, NF-I/B antibody, NF1-B antibody, NFI-B antibody, NFI-RED antibody, NFIB2 antibody, NFIB3 antibody, 6720429L07Rik antibody, E030026I10Rik antibody, nuclear factor I B antibody, nuclear factor 1 B-type antibody, nuclear factor I B L homeolog antibody, nuclear factor I/B antibody, NFIB antibody, LOC100221557 antibody, nfib.L antibody, Nfib antibody
- Background
- NFI-RED, NFIB2, NFIB3, CCAAT-box-binding transcription factor, TGGCA-binding protein
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