This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This EBAG9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human EBAG9.
EBAG9
Reactivity: Mouse
ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Wolf, Reimer, Schuck, Rüder, Gerlach, Müller, Otto, Dörken, Rehm: "Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 4000-19, (2010) (PubMed).
Sonoda, Miyamoto, Nakashima, Wake: "Receptor-binding cancer antigen expressed on SiSo cells induces apoptosis via ectodomain shedding." in: Experimental cell research, Vol. 316, Issue 11, pp. 1795-803, (2010) (PubMed).
Ali-Fehmi, Chatterjee, Ionan, Levin, Arabi, Bandyopadhyay, Shah, Bryant, Hewitt, ORand, Alekseev, Morris, Munkarah, Abrams, Tainsky: "Analysis of the expression of human tumor antigens in ovarian cancer tissues." in: Cancer biomarkers : section A of Disease markers, Vol. 6, Issue 1, pp. 33-48, (2010) (PubMed).
Yang, Zhao, Liao, Zhang, Hu, Dai: "[Evaluation of RCAS1 as serum tumor marker for pancreatic cancer]." in: Zhonghua wai ke za zhi [Chinese journal of surgery], Vol. 47, Issue 13, pp. 999-1001, (2009) (PubMed).
Adamek, Radwańska, Gajda: "Expression of RCAS1 protein in microglia/macrophages accompanying brain tumours. An immunofluorescence study." in: Folia neuropathologica, Vol. 47, Issue 3, pp. 240-6, (2009) (PubMed).
Target
RCAS1 (EBAG9)
(Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.