IRF3 antibody (N-Term)

Catalog No. ABIN1881465

Product Details anti-IRF3 Antibody

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Binding Specificity: AA 46-72, N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: B, Pig

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Irf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the N-terminal region of mouse Irf3.

Clone: RB42372

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-IRF3 antibody (ABIN1881465)

Marichal, Bedoret, Mesnil, Pichavant, Goriely, Trottein, Cataldo, Goldman, Lekeux, Bureau, Desmet: "Interferon response factor 3 is essential for house dust mite-induced airway allergy." in: The Journal of allergy and clinical immunology, Vol. 126, Issue 4, pp. 836-844.e13, (2010) (PubMed).

Menachery, Pasieka, Leib: "Interferon regulatory factor 3-dependent pathways are critical for control of herpes simplex virus type 1 central nervous system infection." in: Journal of virology, Vol. 84, Issue 19, pp. 9685-94, (2010) (PubMed).

Carrigan, Junkins, Yang, Macneil, Richardson, Johnston, Lin: "IFN regulatory factor 3 contributes to the host response during Pseudomonas aeruginosa lung infection in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 6, pp. 3602-9, (2010) (PubMed).

Wang, Basagoudanavar, Wang, Hopewell, Albrecht, García-Sastre, Balachandran, Beg: "NF-kappa B RelA subunit is crucial for early IFN-beta expression and resistance to RNA virus replication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 3, pp. 1720-9, (2010) (PubMed).

Farlik, Reutterer, Schindler, Greten, Vogl, Müller, Decker: "Nonconventional initiation complex assembly by STAT and NF-kappaB transcription factors regulates nitric oxide synthase expression." in: Immunity, Vol. 33, Issue 1, pp. 25-34, (2010) (PubMed).

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: Irf3 (IRF3 Products)

Synonyms: irf3 antibody, IRF3 antibody, C920001K05Rik antibody, IRF-3 antibody, interferon regulatory factor 3 antibody, interferon regulatory factor 7 antibody, irf3 antibody, IRF3 antibody, irf-3 antibody, Irf3 antibody, IRF7 antibody

Background: Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains (By similarity).

Molecular Weight: 46852

NCBI Accession: NP_058545

UniProt: P70671

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades