This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the C-terminal region of human POLR2G.
POLR2G
Reactivity: Human
ELISA
Host: Mouse
Polyclonal
unconjugated
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Cojocaru, Jeronimo, Forget, Bouchard, Bergeron, Côte, Poirier, Greenblatt, Coulombe: "Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription." in: The Biochemical journal, Vol. 409, Issue 1, pp. 139-47, (2007) (PubMed).
Ujvári, Luse: "RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit." in: Nature structural & molecular biology, Vol. 13, Issue 1, pp. 49-54, (2006) (PubMed).
Meka, Werner, Cordell, Onesti, Brick: "Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II." in: Nucleic acids research, Vol. 33, Issue 19, pp. 6435-44, (2005) (PubMed).
Zhou, Deng, Kashanchi, Brady, Shatkin, Kumar: "The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 22, pp. 12666-71, (2003) (PubMed).
Target
POLR2G
(Polymerase (RNA) II (DNA Directed) Polypeptide G (POLR2G))
RPB19 antibody, RPB7 antibody, hRPB19 antibody, hsRPB7 antibody, Rpb7 antibody, POLR2G antibody, 2410046K11Rik antibody, A230108L04Rik antibody, C76415 antibody, RBP7 antibody, Rpo2-7l antibody, RNA polymerase II subunit G antibody, polymerase (RNA) II (DNA directed) polypeptide G antibody, polymerase (RNA) II (DNA directed) polypeptide G L homeolog antibody, POLR2G antibody, Polr2g antibody, polr2g antibody, polr2g.L antibody
Background
This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.