This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This PSMD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-328 amino acids from the C-terminal region of human PSMD4.
Psmd4
Reactivity: Human, Rat, Cow, Sheep, Rabbit, Monkey, Pig, Bat
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Elangovan, Oh, Sukumaran, Wójcik, Yoo: "Functional differences between two major ubiquitin receptors in the proteasome; S5a and hRpn13." in: Biochemical and biophysical research communications, Vol. 396, Issue 2, pp. 425-8, (2010) (PubMed).
Safadi, Shaw: "Differential interaction of the E3 ligase parkin with the proteasomal subunit S5a and the endocytic protein Eps15." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1424-34, (2010) (PubMed).
Zhang, Wang, Ehlinger, Randles, Lary, Kang, Haririnia, Storaska, Cole, Fushman, Walters: "Structure of the s5a:k48-linked diubiquitin complex and its interactions with rpn13." in: Molecular cell, Vol. 35, Issue 3, pp. 280-90, (2009) (PubMed).
Kim, Kim, Uchiki, Gygi, Goldberg: "S5a promotes protein degradation by blocking synthesis of nondegradable forked ubiquitin chains." in: The EMBO journal, Vol. 28, Issue 13, pp. 1867-77, (2009) (PubMed).
Gaurnier-Hausser, Tuszynski: "The immunomodulatory role of angiocidin, a novel angiogenesis inhibitor." in: Current pharmaceutical design, Vol. 15, Issue 17, pp. 1937-48, (2009) (PubMed).
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.