THEM4 antibody (Center)

Catalog No. ABIN1887790

Product Details anti-THEM4 Antibody

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THEM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Purification: Affinity chromatography purified via peptide column

Immunogen: THEM4 antibody was raised against an 18 amino acid peptide near the center of human THEM4.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freezing and thawing repeatly.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term use.Store at -20 °C for long term preservation.

Target Details for THEM4

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Alternative Name: THEM4 (THEM4 Products)

Synonyms: THEM4 antibody, CTMP antibody, 2700077M13Rik antibody, 4921507I02Rik antibody, RGD1304723 antibody, thioesterase superfamily member 4 antibody, thioesterase superfamily member 4 L homeolog antibody, THEM4 antibody, them4 antibody, Them4 antibody, them4.L antibody

Background: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells.Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer.Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase.Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
Synonyms: Thioesterase superfamily member 4, Carboxy-terminal modulator protein, CTMP

NCBI Accession: NP_444283

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway