NCF4 antibody (Internal Region)

Catalog No. ABIN190755

This anti-NCF4 antibody is a Goat Polyclonal antibody detecting NCF4 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for NCF4 antibody (Internal Region) (ABIN190755)

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-NCF4 Antibody

Binding Specificity: Internal Region

Purpose: NCF4 / P40PHOX

Sequence: KDFPEEDDPT N

Specificity: This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2, NP_038202.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx. 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. Th

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Alternative Name: NCF4

Background: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40 kDa , CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)

Gene ID: 4689

NCBI Accession: NP_000622, NP_038202