PAI1 antibody

Catalog No. ABIN191653

This Goat Polyclonal antibody specifically detects PAI1 in IHC (p), IHC (fro), EIA and RIA. It exhibits reactivity toward Human.

Quick Overview for PAI1 antibody (ABIN191653)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAI1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Product Details anti-PAI1 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Purified human PAI-1

Application Details

Application Notes: ELISA. RIA. Immunohistochemistry on Frozen Sections: 1/50-1/100. Immunohistochemistry on Paraffin Sections: 1/50 (Pretreatment with 0.05 % Pronase E for20 minutes recommended).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in aqua bidest to 1 mg/mL.

Buffer: 50 mM TRIS, pH 7.4 containing 0.04 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: SERPINE1 / PAI1

Background: PAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type plasminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder.Synonyms: Endothelial plasminogen activator inhibitor, PAI, PAI-1, PLANH1, Plasminogen Activator Inhibitor 1, Serpin E1

NCBI Accession: NM_000602

UniProt: P05121

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration