CD47 antibody (FITC)

Catalog No. ABIN192129

This anti-CD47 antibody is a Mouse Monoclonal antibody detecting CD47 in FACS. Suitable for Human, Pig and Non-Human Primate. This Primary Antibody has been cited in 1 publication.

Quick Overview for CD47 antibody (FITC) (ABIN192129)

Target: CD47

Reactivity: Human, Pig, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: MEM-122

Product Details anti-CD47 Antibody (FITC)

Purpose: Anti-Hu CD47 FITC

Specificity: The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.

Cross-Reactivity (Details): Non-Human Primates, Human, Porcine

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: COS-7 (African green monkey) cells

Isotype: IgM

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD47 antibody (FITC) (ABIN192129)

Smith, Patel, Seatter, Deehan, Brown, Brooke, Goodridge, Howard, Rigley, Harnett, Harnett: "A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes." in: Blood, Vol. 102, Issue 7, pp. 2532-40, (2003) (PubMed).

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: CD47 Molecule,CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.,IAP, OA3, MER6

Gene ID: 961

UniProt: Q08722