Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody detects endogenous levels of P38MAPK only when phosphorylated at Tyrosine 182.
Purification
Immunoaffinity Chromatography using epitope-specific phosphopeptide.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from Human p38 MAPK around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).
MAPK14
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody (in aliquots) at -20 °C.
Target
MAPK14
(Mitogen-Activated Protein Kinase 14 (MAPK14))