PAK1 antibody (Thr212)

Catalog No. ABIN197365

The Rabbit Polyclonal anti-PAK1 antibody (ABIN197365) specifically detects PAK1 in WB, IF and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PAK1 antibody (Thr212) (ABIN197365)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAK1 Antibody

Binding Specificity: Thr212

Specificity: This antibody AP02681PU detects endogenous levels of total PAK1 protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human PAK1around the phosphorylation site of threonine 212 (P-V-Tp-P-T).

Application Details

Application Notes: Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background: Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK

Gene ID: 5058

NCBI Accession: NP_001122092

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis