IKK alpha antibody (AA 350-400)

Catalog No. ABIN1977815

The Rabbit Polyclonal anti-IKK alpha antibody has been validated for WB, IHC, IF, IP, IHC (p) and PLA. It is suitable to detect IKK alpha in samples from Human, Mouse, Rat, Dog, Rabbit, Horse and Guinea Pig.

Quick Overview for IKK alpha antibody (AA 350-400) (ABIN1977815)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)

Product Details anti-IKK alpha Antibody

Binding Specificity: AA 350-400

Specificity: Region between residue 350 and 400 of human Ikappa-B kinase-alpha using the numbering given in entry NP_001269.3 (GeneID 1147).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:50 - 1:250), IHC, IHC-P (1:100 - 1:500), IP (2 - 5 μg/mg lysate), PLA (1:200 - 1:2000), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline, 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: CHUK / IKKA / IKK Alpha

Background: Name/Gene ID: CHUK
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: CHUK, I-kappa-B kinase alpha, I-kappa-B kinase-alpha, Ikb kinase alpha, IKK-A, IKBKA, IKK1, NFKBIKA, I-kappa-B kinase 1, IkappaB kinase, IKK Alpha, IKK-alpha, IKKA, Transcription factor 16, IkappaBalpha Kinase, IkB kinase alpha subunit, IKK-a kinase, Ikkalpha, TCF-16, TCF16

Gene ID: 1147

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins