HSP27 antibody

Catalog No. ABIN199255

Product Details anti-HSP27 Antibody

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes human HSP27 at 24-27kD. Species cross-reactivity: Chimpanzee and monkey. Does not cross-react with mouse or rat.

Purification: Protein G purified

Immunogen: Partially purified hsp27 protein from breast cancer MCF-7 cells. MW: 24-27kD. Cellular Localization: Cytoplasmic/nuclear.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1-2 μg/mL for 30 minutes at RT. Requires boiling tissue sections in10 mM citrate buffer,  pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes. Positive control: BT474, MDA-MB-453 cells, and breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives, no stabilizing proteins added.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 / HSP27 (HSPB1 Products)

Synonyms: CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

Background: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

Gene ID: 3315

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling