RAD50 antibody (AA 1-425)

Catalog No. ABIN199597

Product Details anti-RAD50 Antibody

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Binding Specificity: AA 1-425

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD50 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes human Rad50.

Purification: Protein A purified

Immunogen: GST fusion protein corresponding to residues 1-425 of human RAD50. The immunogen shares 389/425 identity with mouse and 127/425 identity with yeast.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IP, WB

Usage: Western Blot Analysis: 0.5-2 μg/mL detects RAD50 in a HeLa nuclear lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Rad50 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear lysate. Use 20 μg per lane for minigels. Immunoprecipitation: Reported to immunoprecipitate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Alternative Name: RAD50 (RAD50 Products)

Synonyms: NBSLD antibody, RAD502 antibody, hRad50 antibody, Mrell antibody, Rad50l antibody, 6339 antibody, CG6339 antibody, Dmel\\CG6339 antibody, Dmrad50 antibody, RAD50 antibody, Rad50 antibody, ATRAD50 antibody, DNA REPAIR-RECOMBINATION PROTEIN antibody, F22D22.28 antibody, F22D22_28 antibody, SPAP4C9.01c antibody, im:6906849 antibody, si:ch211-79h18.3 antibody, RAD50 double strand break repair protein antibody, CG6339 gene product from transcript CG6339-RE antibody, RAD50 homolog, double strand break repair protein L homeolog antibody, rad50 antibody, DNA repair protein RAD50 antibody, RAD50 homolog, double strand break repair protein antibody, DNA repair-recombination protein (RAD50) antibody, MRX complex DNA-binding subunit antibody, DNA repair protein Rad50 antibody, RAD50 recombination protein antibody, Rad50 DNA repair/recombination protein antibody, DNA repair protein rad-50 antibody, RAD50 antibody, Rad50 antibody, rad50 antibody, rad50.L antibody, LOC658288 antibody, LOC726642 antibody, rad-50 antibody

Background: Name/Gene ID: RAD50

Synonyms: RAD50, DNA repair protein RAD50, HRad50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Gene ID: 10111

UniProt: Q92878

Pathways: DNA Damage Repair, Protein targeting to Nucleus