CTNNB1 antibody (C-Term)

Catalog No. ABIN200360

Product Details anti-CTNNB1 Antibody

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: Recognizes chicken b-Catenin protein (~92kD). Does not cross-react with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species cross-reactivity: Human & mouse. Reactivity with rat is likely but not confirmed.

Purification: Protein A purified

Immunogen: Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 5-10 μg/mL. Western Blot: 1 μg/mL. Immunoprecipitation: 2-5 μg. Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2-10 μg/mL. Requires heat induced epitope retrieval. Positive control: HeLa, A431 and WI-38 human cell lines.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: CTNNB1 / Beta Catenin (CTNNB1 Products)

Synonyms: Jup antibody, LOC100217813 antibody, CTNNB1 antibody, CTNNB antibody, Bfc antibody, Catnb antibody, Mesc antibody, ctnnb antibody, id:ibd2058 antibody, wu:fb73e10 antibody, wu:fi81c06 antibody, wu:fk25h01 antibody, ctnnb1 antibody, CHBCAT antibody, MRD19 antibody, armadillo antibody, beta-catenin antibody, catenin beta 1 antibody, catenin (cadherin associated protein), beta 1 antibody, catenin (cadherin-associated protein), beta 1 antibody, catenin beta 1 S homeolog antibody, catenin beta 1 L homeolog antibody, CTNNB1 antibody, Ctnnb1 antibody, ctnnb1 antibody, ctnnb1.S antibody, ctnnb1.L antibody

Background: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gene ID: 1499

UniProt: P35222

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2