Cytokeratin 7 antibody

Catalog No. ABIN200763

Product Details anti-Cytokeratin 7 Antibody

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes human Cytokeratin 7 (exclusively). Reacts with a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.

Purification: Protein G purified

Immunogen: Cytokeratin 7.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), ICC, IHC, IHC-Fr, IHC-P, WB (1:50 - 1:500)

Usage: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. This antibody reacts particularly well in paraffin embedded tissue. Flow Cytometry: 1:50-1:100. Immunohistochemistry: Frozen tissue after fixation in methanol. Paraffin embedded tissue sections pretreated with 0.1 % pronase/ 0.1 % trypsin in PBS or 0.5 % pepsin in 0.01N HCl. Avidin-biotinylated horseradish peroxidase complex as a detection reagent. 1:50-1:100 (ABC). Western Blot: 1:50-1:500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide, 40 % glycerol. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: KRT7 / CK7 / Cytokeratin 7 (KRT7 Products)

Synonyms: CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody

Background: Name/Gene ID: KRT7

Synonyms: KRT7, CK-7, Cytokeratin 7, CK7, K2C7, K7, Keratin 7, Keratin-7, SCL, Type II mesothelial keratin K7, Cytokeratin-7, Sarcolectin, Type-II keratin Kb7

Gene ID: 3855