NOS1 antibody (AA 724-739)
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- Target See all NOS1 Antibodies
- NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
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Binding Specificity
- AA 724-739
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NOS1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
- Specificity
- Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.
- Purification
- Affinity purified
- Immunogen
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Ved in human, rat and mouse. IMMUNOGEN: Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product NOS1 Primary Antibody
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- Application Notes
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Approved: ELISA, IF, IHC, WB (1 - 10 μg/mL)
Usage: Immunohistochemistry: Use at 5-10 μg/mL on rat neuronal sections fixed with 3.5 % paraformaldehyde. Incubation overnight at 4?C with a 1:100-1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 1-10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155kD protein. ELISA: Use at 1.0 μg/mL or lower on plates coated with 50-100 ng of peptide antigen. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.1 % BSA, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
- Alternative Name
- NOS1 / nNOS (NOS1 Products)
- Synonyms
- NOS1 antibody, IHPS1 antibody, N-NOS antibody, NC-NOS antibody, NOS antibody, bNOS antibody, nNOS antibody, 2310005C01Rik antibody, NO antibody, NOS-I antibody, Nos-1 antibody, nos antibody, ihps1 antibody, nnos antibody, nos1 antibody, nos1-A antibody, nanos C2HC-type zinc finger 1 antibody, nitric oxide synthase 1 antibody, nitric oxide synthase 1, neuronal antibody, nitric oxide synthase 1 (neuronal) antibody, nitric oxide synthase 1 L homeolog antibody, NANOS1 antibody, NOS1 antibody, Nos1 antibody, nos1 antibody, nos1.L antibody
- Background
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Name/Gene ID: NOS1
Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i - Gene ID
- 4842
- UniProt
- P29475
- Pathways
- Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction
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