NOS1 antibody (pSer1417)

Catalog No. ABIN201345

Product Details anti-NOS1 Antibody

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Binding Specificity: pSer1417

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes human phosphorylated nNOS/NOS I, Mr 170kD. Species cross-reactivity: rat. Species sequence Homology: mouse, zebrafish and frog. Sequence homology: Ser1451 of the nNOSu isoform.

Purification: Immunoaffinity purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids surrounding Ser1417 of human neuronal Nitric Oxide Synthase (nNOS), with an N-terminal cysteine added for conjugation purposes, where pS is phosphorylated serine corresponding to the AMPK phosphorylation site. This site also corresponds to Ser1451 of the nNOSu isoform. The immunizing sequence is identical in mouse, rat, zebrafish and frog.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.175M sodium chloride, 0.07 % sodium azide, before the addition of glycerol to 30 %.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1 / nNOS (NOS1 Products)

Synonyms: NOS1 antibody, IHPS1 antibody, N-NOS antibody, NC-NOS antibody, NOS antibody, bNOS antibody, nNOS antibody, 2310005C01Rik antibody, NO antibody, NOS-I antibody, Nos-1 antibody, nos antibody, ihps1 antibody, nnos antibody, nos1 antibody, nos1-A antibody, nanos C2HC-type zinc finger 1 antibody, nitric oxide synthase 1 antibody, nitric oxide synthase 1, neuronal antibody, nitric oxide synthase 1 (neuronal) antibody, nitric oxide synthase 1 L homeolog antibody, NANOS1 antibody, NOS1 antibody, Nos1 antibody, nos1 antibody, nos1.L antibody

Background: Name/Gene ID: NOS1

Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i

Gene ID: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction