Phospholamban antibody (pSer16)

Catalog No. ABIN204571

Product Details anti-Phospholamban Antibody

Target: Phospholamban (PLN)

Binding Specificity: AA 14-25, pSer16

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: Recognizes human Phospholamban phosphorylated (Ser16) (Mr 5-25kD). Species cross-reactivity: bovine. Species sequence Homology: mouse, rat, rabbit, porcine and canine.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa14-25 of human cardiac phospholamban with a cysteine added at the C-terminal (RA[pS]TIEMPQQAR-C) (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. Additional non-specific bands may be detected in some lysates (Mr 75 & 100kD).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: PLN / Phospholamban (PLN Products)

Synonyms: LOC100226261 antibody, plb antibody, cmd1p antibody, CMD1P antibody, CMH18 antibody, PLB antibody, Plb antibody, Plm antibody, phospholamban antibody, PLN antibody, pln antibody, Pln antibody

Background: Name/Gene ID: PLN

Synonyms: PLN, CMD1P, CMH18, PLB, Cardiac phospholamban, Phospholamban

Gene ID: 5350

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity