HLAG antibody

Catalog No. ABIN206999

The Mouse Monoclonal anti-HLAG antibody is suitable to detect HLAG in samples from Human. It has been validated for WB, IHC and IHC (fro).

Quick Overview for HLAG antibody (ABIN206999)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLAG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-G-1

Product Details anti-HLAG Antibody

Specificity: Reacts with denatured HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells

Purification: Protein A purified

Immunogen: Recombinant human HLA-G denatured heavy chain.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB (1:60 - 1:100)

Usage: Immunohistochemistry (paraffin sections): Recommended dilution - 1:60 to 1:100. Incubation: 1 h at RT. Positive tissue - Human placenta - extravillous cytotrophoblast. Staining technique: Standard ABC technique. Pretreatment: Heat retrieval in 0.01M citra

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G

Background: Name/Gene ID: HLA-G

Synonyms: HLA-G, HLAG, HLA-6.0, MHC class I antigen G, HLA G antigen

Gene ID: 3135

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response