MYB antibody (C-Term)

Catalog No. ABIN213216

Product Details anti-MYB Antibody

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYB antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes mouse Myb, Mr ~80kD, containing C-terminal amino acids from 401-636. Species cross-reactivity: human.

Purification: Ion exchange chromatography

Immunogen: Recombinant murine Myb purified by SDS.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-2 μg/mL detects Myb in 20 μg of RIPA lysate from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myb (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg of a previous lot immunoprecipitated Myb from Jurkat cell lysate.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MYB

Target: MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

Alternative Name: MYB / c-Myb (MYB Products)

Synonyms: Cmyb antibody, c-myb antibody, c-myb_CDS antibody, efg antibody, AI550390 antibody, M16449 antibody, cmyb antibody, xmyb antibody, MYB proto-oncogene, transcription factor antibody, myeloblastosis oncogene antibody, MYB proto-oncogene, transcription factor S homeolog antibody, v-myb avian myeloblastosis viral oncogene homolog antibody, MYB antibody, Myb antibody, myb.S antibody, myb antibody

Background: Name/Gene ID: MYB

Synonyms: MYB, C-myb_CDS, C-myb8B_CDS, C-myb protein (140 AA), C-myb10A_CDS, C-myb13A_CDS, Efg, Transcriptional activator Myb, C-myb, C-myb14A_CDS, Cmyb, Proto-oncogene c-Myb

Gene ID: 4602

UniProt: P10242

Pathways: Chromatin Binding, Positive Regulation of Immune Effector Process