HTR1F antibody (Cytoplasmic Domain)

Catalog No. ABIN213590

Product Details anti-HTR1F Antibody

Target: HTR1F (5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR1F antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human 5HT1F Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon (100%) Gorilla, Monkey, Bovine, Elephant, Horse, Rabbit, Guinea pig, Platypus (94%) Dog, Bat (88%) Rat, Panda (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT1F Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon (100%), Gorilla, Monkey, Bovine, Elephant, Horse, Rabbit, Guinea pig, Platypus (94%), Dog, Bat (88%), Rat, Panda (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (6.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 6.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HTR1F

Target: HTR1F (5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F))

Alternative Name: HTR1F / 5-HT1F Receptor (HTR1F Products)

Synonyms: 5-HT-1F antibody, 5-HT1F antibody, 5HT6 antibody, HTR1EL antibody, MR77 antibody, Htr1eb antibody, 5htr1e antibody, 5htr1f antibody, 5-hydroxytryptamine receptor 1F antibody, 5-hydroxytryptamine (serotonin) receptor 1F antibody, uncharacterized LOC100406194 antibody, HTR1F antibody, Htr1f antibody, LOC100406194 antibody

Background: Name/Gene ID: HTR1F
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR1F, 5-HT1F, 5-HT1f receptor, 5HT1F Receptor, 5-HT-1F, 5HT6, HTR1EL, MR77, Serotonin 1f receptor, Serotonin 5-HT-1f receptor, Serotonin receptor 1F

Gene ID: 3355

Pathways: JAK-STAT Signaling