HSF1 antibody (C-Term)

Catalog No. ABIN6736730

Product Details anti-HSF1 Antibody

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Hamster, Dog, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HSF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Pig (100%) Rabbit (92%) Mouse, Rat, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human HSF1 (Q00613, NP_005517). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Hamster, Zebu, Dog, Bovine, Horse, Pig (100%), Marmoset, Panda, Rabbit, Platypus (92%), Mouse, Rat, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.06 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1 (HSF1 Products)

Synonyms: hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody

Background: Name/Gene ID: HSF1

Synonyms: HSF1, HSTF 1, Heat shock factor protein 1, HSF 1, HSTF1

Gene ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613