MCM2 antibody (AA 21-31)

Catalog No. ABIN214365

Product Details anti-MCM2 Antibody

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Binding Specificity: AA 21-31

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Brand: IHC-plus™

Specificity: Amino acids 21-31 of human MCM2 protein (see below).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human MCM2.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2 (MCM2 Products)

Synonyms: CG7538 antibody, DmMCM2 antibody, DmMcm2 antibody, DmeMCM2 antibody, Dmel\\CG7538 antibody, MCM2 antibody, MCM2-7 antibody, MCM2_DROME antibody, McM2 antibody, PCR3 antibody, dMCM2 antibody, l(3)rL074 antibody, XMcm2 antibody, bm28 antibody, ccnl1 antibody, cdc19 antibody, cdcl1 antibody, mitotin antibody, cb737 antibody, chunp6905 antibody, BM28 antibody, CCNL1 antibody, CDCL1 antibody, D3S3194 antibody, MITOTIN antibody, MCM7 antibody, AA959861 antibody, AW476101 antibody, Mcmd2 antibody, mKIAA0030 antibody, Minichromosome maintenance 2 antibody, minichromosome maintenance complex component 2 antibody, minichromosome maintenance complex component 2 L homeolog antibody, Mcm2 antibody, mcm2 antibody, MCM2 antibody, mcm2.L antibody

Background: Name/Gene ID: MCM2

Synonyms: MCM2, BM28, Cell devision cycle-like 1, D3S3194, Cdc19, Cyclin-like 1, KIAA0030, Nuclear protein BM28, CDCL1, MITOTIN

Gene ID: 4171

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA