GRP78 antibody (AA 649-654)

Catalog No. ABIN215160

Product Details anti-GRP78 Antibody

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Binding Specificity: AA 649-654

Reactivity: Rat, Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Identifies purified Grp78 (BiP) protein and reacts with proteins corresponding to the apparent molecular mass of Grp78 and Grp94 on SDS-PAGE immunoblots in samples from mammal, bird, insect and plant origin. Cross reacts with yeast proteins containing the HDEL sequence. An unidentified protein at ~55kD is also detected in some lysates. This antibody does not react with Hsp70 (Hsp72) or Hsc70 (Hsp73).

Purification: Protein G purified

Immunogen: A 6 residue synthetic peptide, SEKDEL, based on the rat Grp78 (residues 649-654), and the peptide coupled to KLH.

Type of Immunogen: Synthetic peptide - KLH conjugated

Clone: 10C3

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC (5 μg/mL), IHC (1:400), IP (5 μg/mL), WB

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (Colorimetric): 4 μg/mL. Immunoprecipitation: 5 μg/mL. Immunocytochemistry: 5 μg/mL. Immunohistochemistry: 1:400. Positive control: HeLa Heat Shocked Cell Lysate.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5 / GRP78 / BIP (HSPA5 Products)

Synonyms: GRP78 antibody, BiP antibody, GRP-78 antibody, grp78 antibody, hspa5a antibody, BIP antibody, MIF2 antibody, AL022860 antibody, AU019543 antibody, Bip antibody, D2Wsu141e antibody, D2Wsu17e antibody, Grp78 antibody, Hsce70 antibody, SEZ-7 antibody, Sez7 antibody, baffled antibody, mBiP antibody, cb865 antibody, fb60h09 antibody, fi36d04 antibody, wu:fb60h09 antibody, wu:fi36d04 antibody, zgc:55994 antibody, zgc:77606 antibody, 78 kDa glucose-regulated protein antibody, heat shock protein family A (Hsp70) member 5 antibody, BiP/GRP78 antibody, glucose-regulated protein 78 antibody, putative glucose-regulated protein 78 antibody, Hsp70 family ATPase KAR2 antibody, heat shock protein family A (Hsp70) member 5 S homeolog antibody, heat shock 70 kDa protein 5a antibody, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody, heat shock protein 5 antibody, heat shock protein family A member 5 antibody, CpipJ_CPIJ003550 antibody, HSPA5 antibody, grp78 antibody, LOC100533358 antibody, BiP/grp78 antibody, Tc00.1047053506585.40 antibody, Tb11.02.5450 antibody, Tb11.02.5500 antibody, LMJF_28_1200 antibody, KAR2 antibody, LOC100135840 antibody, hspa5.S antibody, hspa5 antibody, Hspa5 antibody

Background: Name/Gene ID: HSPA5

Synonyms: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78

Gene ID: 3309

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling