HPV16 E7 antibody (Middle Region) (HRP)

Catalog No. ABIN2173806

Product Details anti-HPV16 E7 Antibody (HRP)

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Binding Specificity: Middle Region

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPV16 E7 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody may also detect HPV35-E7

Cross-Reactivity: Human, Virus

Cross-Reactivity (Details): HPV16

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from middle of HPV16-E7

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HPV16 E7

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Abstract: HPV-16 E7 Products

Synonyms: transforming protein E7 antibody, E7 antibody

Target Type: Viral Protein

Background:

Synonyms: Protein E7, E7

Background: Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.

Gene ID: 1489079

UniProt: P03129