Mbl1 antibody

Catalog No. ABIN2191774

Product Details anti-Mbl1 Antibody

Target: Mbl1 (Mannose-Binding Lectin (Protein A) 1 (Mbl1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Mbl1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: 2B4

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (2B4 is a calcium- dependent antibody).

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Mbl1 antibody (ABIN2191774)

Liu, Jensen, Hansen, Petersen, Takahashi, Ezekowitz, Hansen, Jensenius, Thiel: "Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses." in: Scandinavian journal of immunology, Vol. 53, Issue 5, pp. 489-97, (2001) (PubMed).

Sastry, Zahedi, Lelias, Whitehead, Ezekowitz: "Molecular characterization of the mouse mannose-binding proteins. The mannose-binding protein A but not C is an acute phase reactant." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 2, pp. 692-7, (1991) (PubMed).

Target Details for Mbl1

Target: Mbl1 (Mannose-Binding Lectin (Protein A) 1 (Mbl1))

Alternative Name: Mannose Binding Lectin A (Mbl1 Products)

Synonyms: MBL-A antibody, MBP-A antibody, pMBP-28 antibody, S-MBP antibody, Mbpa antibody, Mlb1 antibody, mannan-binding lectin (A) antibody, mannose-binding lectin (protein A) 1 antibody, MBL1 antibody, Mbl1 antibody

Background: Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N- acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 2B4 is a calcium-dependent antibody.