Complement Receptor 1 & 2 antibody

Catalog No. ABIN2192213

Product Details

Target: Complement Receptor 1 & 2

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunofluorescence (IF)

Sterility: 0.2 μm filtered

Clone: 7E9

Application Details

Application Notes: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Hoefer, Aichem, Knight, Illges: "Modulation of murine complement receptor type 2 (CR2/CD21) ectodomain shedding by its cytoplasmic domain." in: Molecular immunology, Vol. 45, Issue 8, pp. 2127-37, (2008) (PubMed).

Pramoonjago, Takeda, Kim, Inoue, Kinoshita: "Ligand specificities of mouse complement receptor types 1 (CR1) and 2 (CR2) purified from spleen cells." in: International immunology, Vol. 5, Issue 4, pp. 337-43, (1993) (PubMed).

Kinoshita, Thyphronitis, Tsokos, Finkelman, Hong, Sakai, Inoue: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, (1991) (PubMed).

Kinoshita, Takeda, Hong, Kozono, Sakai, Inoue: "Monoclonal antibodies to mouse complement receptor type 1 (CR1). Their use in a distribution study showing that mouse erythrocytes and platelets are CR1-negative." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 9, pp. 3066-72, (1988) (PubMed).

Target Details

Target: Complement Receptor 1 & 2

Background: The monoclonal antibody 7E9 recognizes mouse complement receptors type 1 (CR1) and 2 (CR2). CR1 and CR2 are cell surface glycoproteins that are capable of binding to activation fragments of the third and/or fourth complement components (C3 and/or C4). They play a role in the clearance of immune- complexes, phagocytosis, complement regulation, and immunoregulation. Mouse CR1 (MCR1, 190 kD) is found on the surface of B-lymphocytes, follicular dendritic cells and at lower levels on peritoneal macrophages and activated granulocytes. MRC1 has binding activity for C3b and serves as a cofactor for factor I-mediated cleavage of C3b. Mouse CR2 (MCR2, 150 kD) is a type I transmembrane glycoprotein that binds complement fragments (C3d(g), iC3b) and interferon (IFN)-alpha. MCR2 is expressed on B-lymphocytes and probably on follicular dendritic cells. On human B lymphocytes it acts as the Epstein-Barr virus (EBV) receptor. MCR2 mediates the formation of rosettes between B-lymphocytes and E-bearing Crd. MCR1 and MRC2 are very closely related. They are both products of a single gene, Cr2, formed by alternative splicing of mRNA. MCR2 corresponds to the carboxy-terminal portion of MCR1. This is in contrast with human CR1 (CD35) and CR2. The monoclonal antibody 7E9 does not inhibit rosette formation between 2PK3 cells and Crd-coated SRBC indicator cells. CR1, CD35, KN Aliases CR2, CD21 Purified mouse CR1 from serum Immunogen Rat IgG2a