IKK alpha antibody (N-Term)

Catalog No. ABIN220382

The Rabbit Polyclonal anti-IKK alpha antibody has been validated for WB, ELISA, IP and ICC. It is suitable to detect IKK alpha in samples from Human, Mouse, Rat and Cow.

Quick Overview for IKK alpha antibody (N-Term) (ABIN220382)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-IKK alpha Antibody

Binding Specificity: N-Term

Specificity: Detects total IKK alpha proteins. Detects IKK alpha only. Does not cross-react with IKK beta or IKK gamma. Species cross-reactivity: Human, mouse, and rat. Predicted to cross-react with bovine based on sequence homology.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to the 20 N-terminal amino acids of human IKK alpha (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IP (1:500), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:500. Immunocytochemistry (ABC): 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: CHUK / IKKA / IKK Alpha

Background: Name/Gene ID: CHUK
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: CHUK, I-kappa-B kinase alpha, I-kappa-B kinase-alpha, Ikb kinase alpha, IKK-A, IKBKA, IKK1, NFKBIKA, I-kappa-B kinase 1, IkappaB kinase, IKK Alpha, IKK-alpha, IKKA, Transcription factor 16, IkappaBalpha Kinase, IkB kinase alpha subunit, IKK-a kinase, Ikkalpha, TCF-16, TCF16

Gene ID: 1147

UniProt: O15111

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins