Prohibitin antibody

Catalog No. ABIN221967

Product Details anti-Prohibitin Antibody

Target: Prohibitin (PHB)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: Recognizes rat Prohibitin at 30kD. Species cross-reactivity: Human, porcine, mouse and chicken.

Purification: Protein G purified

Immunogen: Purified recombinant rat prohibitin protein. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate., (Protein G). Native and denatured. Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: MAD109, or PC12 cells, heart.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: PHB / Prohibitin (PHB Products)

Synonyms: phb1 antibody, MGC75944 antibody, PHB antibody, Phb antibody, ACYPI000080 antibody, phb antibody, PHB1 antibody, Bap32 antibody, sr:nyz128 antibody, wu:fa18f07 antibody, wu:fb09f05 antibody, prohibitin1 antibody, xphb1 antibody, prohibitin antibody, prohibitin-2 antibody, prohibitin L homeolog antibody, PHB antibody, phb antibody, LOC484431 antibody, LOC5576054 antibody, Phb antibody, phb.L antibody

Background: Name/Gene ID: PHB

Synonyms: PHB, PHB1, Prohibitin

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling