BAD antibody (pSer136)

Catalog No. ABIN222319

This Rabbit Polyclonal antibody specifically detects BAD in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BAD antibody (pSer136) (ABIN222319)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAD Antibody

Binding Specificity: pSer136

Specificity: Detects overexpressed levels of Bad only when phosphorylated at Ser136. Does not detect non-phosphorylated Bad or Bad phosphorylated at other sites. Does not detect related family members. Species cross-reactivity: Human, mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues around Ser136 of mouse Bad.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:500)

Usage: Suitable for use in Western Blot. Western Blot: 1:500. Diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20. Incubate at 4?C overnight with gentle shaking.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD

Background: Name/Gene ID: BAD
Family: Apoptosis

Synonyms: BAD, BBC2, Bcl-2-like protein 8, BCL2-binding component 6, BCL2L8, BBC6, BCL-X/BCL-2 binding protein, Bcl-2-binding component 6, Bcl2 antagonist of cell death, BCL2-binding protein, Bcl2-L-8

Gene ID: 572

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events