BAD antibody (pSer155)

Catalog No. ABIN222326

The Rabbit Polyclonal anti-BAD antibody has been validated for WB and ELISA. It is suitable to detect BAD in samples from Human and Mouse.

Quick Overview for BAD antibody (pSer155) (ABIN222326)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BAD Antibody

Binding Specificity: pSer155

Specificity: Detects overexpressed levels of Bad only when phosphorylated at Ser155. Does not detect Bad phosphorylated at other sites or related family members. Species cross-reactivity: Human.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser155 of mouse Bad.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:1000), WB (1:1000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. ELISA: 1:1000.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD

Background: Name/Gene ID: BAD
Family: Apoptosis

Synonyms: BAD, BBC2, Bcl-2-like protein 8, BCL2-binding component 6, BCL2L8, BBC6, BCL-X/BCL-2 binding protein, Bcl-2-binding component 6, Bcl2 antagonist of cell death, BCL2-binding protein, Bcl2-L-8

Gene ID: 572

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events