HRAS antibody (AA 62-76)

Catalog No. ABIN224929

This Rat Monoclonal antibody specifically detects HRAS in WB, IHC, IF, IP, IHC (p), IHC (fro) and Neut. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HRAS antibody (AA 62-76) (ABIN224929)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Neutralization (Neut)

Product Details anti-HRAS Antibody

Binding Specificity: AA 62-76

Specificity: Recognizes phosphorylated and non-phosphorylated human, rat and mouse ras. Does not cross-react with the rap protein. Neutralizes the biological and biochemical activities by binding to residues E63, S65, A66, M67, Q70 and R73. Positive Controls: SW480 or Y1 cells (IF and IP), normal skin (IHC, frozen and paraffin), A431, Hela or ML-1 cells (WB).

Purification: Purified

Immunogen: Recombinant p21 ras protein. Epitope: aa62-76 of human ras.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF (2.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, Neut, WB (2.5 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Immunofluorescence: 2.5 μg/mL. Western Blot (chemiluminescence detection): 2.5 μg/mL. Immunohistochemistry (Frozen): 5 μg/mL. Immunohistochemistry: 1-5 μg/mL. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize. Immunoprecipitation (autoradiographic detection): 1 μg/sample SW480 or Y1 cells. Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS / H-Ras

Background: Name/Gene ID: HRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21ras, RASH1, C-BAS/HAS, C-ha-ras, p19 H-RasIDX protein

Gene ID: 3265

UniProt: P01112

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling