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Nestin antibody

NES Reactivity: Human WB, IHC, ELISA, ICC, IHC (p), IHC (fro) Host: Mouse Monoclonal unconjugated

Catalog No. ABIN228953

Datasheet as PDF

Target See all Nestin (NES) Antibodies

Nestin (NES)
Reactivity
119

67

46

4

3

2

2

2

1

1

1

1
Human
Host
94

33

3

1

1
Mouse
Compatible Secondaries
Clonality
99

33
Monoclonal
Conjugate
57

10

8

7

7

4

4

4

4

4

4

4

3

3

3

3

3
This Nestin antibody is un-conjugated
Application
69

46

46

46

40

39

34

24

23

14

7

6

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

Reacts exclusively with the human nestin protein. Species cross-reactivity: Does not cross-react with rodent Nestin.

Purification

Protein G purified

Immunogen

150-amino-acid fragment from cloned human nestin conjugated to glutathione S-transferase.

Isotype

IgG1

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NES Reactivity: Human WB, IHC, ELISA, FACS, IF Host: Mouse Monoclonal 2C2F7 unconjugated

anti-Nestin (NES) (AA 1484-1500) antibody
NES Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) (AA 252-290), (N-Term) antibody
NES Reactivity: Human, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) antibody
NES Reactivity: Mouse IHC, ICC Host: Rat Monoclonal 7A3 unconjugated

anti-Nestin (NES) (AA 698-844) antibody
NES Reactivity: Human IHC (f) Host: Mouse Monoclonal NES-2911 unconjugated

anti-Nestin (NES) (AA 1389-1416) antibody
NES Reactivity: Human WB, IHC, ELISA, FACS, IF Host: Rabbit Polyclonal unconjugated

anti-Nestin (NES) antibody (FITC)
NES Reactivity: Human IF, ICC Host: Mouse Monoclonal 10C2 FITC

Application Notes

Approved: ELISA, ICC (1:50 - 1:100), IHC, IHC-Fr, IHC-P, WB (1:500 - 1:2500)

Usage: Suitable for use in Immunocytochemistry, Immunohistochemistry, ELISA, and Western blot. Immunohistochemistry: 1:50-1:100. Frozen and paraffin-embedded tissues with a fluorescein or rhodamine conjugated anti-mouse secondary antibody. Immunocytochemistry: 1:50-1:100 with a fluorescein or rhodamine conjugated anti-mouse secondary antibody. Western Blot: 1:500-1:2500 using chemiluminescent detection.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 0.09 % sodium azide, 40 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Target

Nestin (NES)

Alternative Name

Nestin (NES Products)

Synonyms

paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Background

Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gene ID

10763

UniProt

P48681