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14-3-3 sigma/SFN antibody

SFN Reactivity: Human WB, IHC, IF, IP, IHC (p) Host: Mouse Monoclonal unconjugated

Catalog No. ABIN229459

Datasheet as PDF

Target See all 14-3-3 sigma/SFN (SFN) Antibodies

14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
Reactivity
102

35

28

5

5

5

4

4

4

4

3

1

1

1

1

1
Human
Host
93

8

2

1
Mouse
Compatible Secondaries
Clonality
88

16
Monoclonal
Conjugate
47

11

9

6

4

4

3

3

3

3

3

3

1

1

1

1

1
This 14-3-3 sigma/SFN antibody is un-conjugated
Application
85

44

37

22

16

15

14

13

13

8

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

Recognizes human 14-3-3 sigma protein (26-30kD). Does not cross-react with other isoforms of 14-3-3.

Purification

Protein G purified

Immunogen

Recombinant human 14-3-3 sigma protein (26-30kD). Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotype

IgG1 kappa

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Verified SFN Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Goat Polyclonal unconjugated

anti-Stratifin (SFN) (AA 1-248) antibody
SFN Reactivity: Human WB, ELISA, IP, IHC (p) Host: Mouse Monoclonal 3C3 unconjugated

anti-Stratifin (SFN) (C-Term) antibody
SFN Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Stratifin (SFN) (AA 140-156) antibody
SFN Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Stratifin (SFN) (AA 1-248) antibody
SFN Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Stratifin (SFN) (AA 222-248), (C-Term) antibody
SFN Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB27523 unconjugated

anti-Stratifin (SFN) (AA 1-248) antibody
SFN Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 2G2 unconjugated

anti-Stratifin (SFN) antibody
SFN Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal CPTC-SFN-2 unconjugated

anti-Stratifin (SFN) (AA 1-249) antibody
SFN Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 3C3 unconjugated

Application Notes

Approved: IF, IHC, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate. Use Protein G. Western Blot: 1 μg/mL for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: HT29 cells and skin.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Supplied as a liquid in PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Target

14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name

SFN / Stratifin / 14-3-3 Sigma (SFN Products)

Synonyms

YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody

Background

Name/Gene ID: SFN

Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS

Gene ID

2810

UniProt

P31947

Pathways

p53 Signaling, Myometrial Relaxation and Contraction