Collagen Type I antibody

Catalog No. ABIN238357

This Rabbit Polyclonal antibody specifically detects Collagen Type I in IHC (fro), IF, IHC (p), RIA and EIA. It exhibits reactivity toward Rat.

Quick Overview for Collagen Type I antibody (ABIN238357)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Specificity: Rat Collagen type I: 100 % Rat Collagen II, III and V: < 0.1 % Human, Mouse, Chicken Collagen type I: < 0.1 % Rat Elastin: < 0.1 % (determined by RIA at 1/200 dilution).

Purification: Affinity Chromatography

Immunogen: Purified Collagen type I from Rat skin

Application Details

Application Notes: ELISA: >/= 1: 200. RIA. Immunofluorescence: 1: 80. Immunohistochemistry on paraffin sections: 1: 500 (60 min at RT or 2-8C over night). Positive control: Rat skin. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS, None, None

Precaution of Use: Do not use Sodium Azide as Preservative.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is mainly found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 29393

NCBI Accession: NP_445756

UniProt: P02454